Animal Hospital Second Floor Update: How to Unlock the Lab and Make DNA Vials

The Second Floor update opens a whole new level above the wards in Animal Hospital, and most of it exists to support one system: pulling DNA out of your patients and turning it into experimental vials. There is also a break room, a hidden hatch, and a piece of lore that hints at rooms the hospital sealed off years ago.

Quick answer: Once you have had your first ambulance visit (or you started the run with Fast Start), head to the stairs marked “UPPER FLOOR” around Aisles 6 to 8, grab the DNA Sampler in the Lab, sample a patient, then run the sample through the Sequencer and the Vial Press to get a finished vial.

Animal Hospital hospital interior during a shift after the Second Floor update — The Second Floor update adds an upper level with a Lab and a break room above the main ward. Image: Animal Anomaly

How to unlock the second floor and where the stairs are

The upper floor is gated behind your first ambulance visit. If you skip the tutorial with Fast Start when you deploy, the floor is available from the opening shift instead, which is the quickest route if you only want to test the Lab. The experience page on Roblox is where you launch into a session.

The stairwell sits in the corridors around Aisles 6 to 8 and is signposted “UPPER FLOOR” above the door. Walk up to the handle and take the on-screen prompt to open it, then climb. There is no key or code involved.

Door handle in the hospital corridor showing the E Open interaction prompt during Shift 1 — Doors on the route to the stairs use the standard open prompt on the handle. Image: Animal Anomaly

Two rooms wait at the top. One is a staff break room with a “Run Fast” vending machine and a bulletin board of sticky notes, including complaints about a new intern with smelly feet and a countdown reading “4 Days before I can go home…” The other is the Lab, and that is where everything mechanical happens.

What is inside the Lab

The Lab holds three pieces of equipment you need to learn. The DNA Sampler is the handheld tool you carry down to patients, the Sequencer grows a sample into a culture, and the Vial Press turns that finished culture into a usable vial. Around the walls are ingredient shelves labelled with the four DNA letters, and each letter dispenses a different feed for the culture.

Shelf	Ingredient
T	Purple mushrooms
A	Pink jelly
G	Milk
C	Ginger

How to make a DNA vial

Pick up the DNA Sampler from the Lab counter. The tool reads “sampler: ready” when it is charged and able to take a sample, so check that before you walk back downstairs.

Find a patient to sample. You need someone actually checked in and occupying a room, so work a normal reception cycle first and let visitors come in as usual.

Waiting room patient saying I'm here for my appointment with the Wait for visitors objective active — You need a checked-in patient before the sampler will do anything. Image: Animal Anomaly

Use the DNA Sampler on the patient to draw the sample. If you try to use the syringe without having taken a sample first, the game blocks the action and tells you to sample a patient beforehand.

On-screen sampler warning telling the player to take a sample from a patient before using the syringe — The sampler warning that appears if you reach for the syringe with no sample stored. Image: Animal Anomaly

Carry the sample back up to the Lab and insert it into the Sequencer. A minigame starts, and you clear it by clicking while the marker sits inside the green section of the DNA gauge.

Feed the growing culture using the lettered shelves. Each ingredient you add extends the chain, and combinations matter — a run of A-A-A, for example, can grow a stable culture. Not every sequence works, so treat the first few attempts as testing.

Take the completed culture to the Vial Press and press it. Collect the vial that pops out, then return the DNA Sampler to the Lab so it is available for the next sample.

Objective reading you return the dna sampler as the player places it back on the laboratory counter — Putting the DNA Sampler back on the Lab counter closes out the objective. Image: Animal Anomaly

If the culture goes unstable

An unstable chain cannot be salvaged mid-process. You have to start the whole sequence again from a fresh sample, which means another trip down to a patient with the sampler. Keep track of the letter order you fed in so you are not repeating a combination that already failed.

What DNA vials do to patients

Finished vials are used on patients, and the result depends on the serum you produced. Effects include shrinking a patient down to a tiny size, making hair sprout on them, and knocking them out cold. Since fainted patients create work for you, be deliberate about who you test an unknown serum on during a busy shift.

The hidden hatch and the clown jail

One corner of the Lab floor has a hatch built into it. Drop through and you land in a small jail holding a clown-like NPC. Talk to him and he rambles about balloons, including a claim about spending eighteen years with balloons and the Laboratory.

He eventually vanishes and leaves an Animal News item behind. Picking it up completes the “Clown” discovery, so that is your confirmation the secret registered. The newspaper reports that the Laboratory and several patient rooms were closed and sealed with no reopening date given, alongside a story about a fatal collision between a passenger car and a Nom Aly Burgers delivery truck.

Note: the room has a second exit that comes out at the new locker in the first-floor receptionist room. Once you know it is there, you can move between reception and the Lab without using the stairs at all, which saves a lot of time when a shift gets hectic.

Everything else in the Second Floor update

Change	Detail
Effects board	A new board that displays stackable effects
Hidden location	The sealed jail beneath the Lab
Lore	New lore elements, including the Animal News article
Achievements	New achievements added
Skins	Four new skins
Maintenance	Bug fixes and a leaderboard reset

The DNA system is the part worth spending real time on, because the combinations are something you work out yourself rather than an item you simply collect. And the newspaper’s mention of wards that were sealed rather than demolished leaves an obvious door open for whatever gets unlocked next.